MALDI-TOF mass spectrometry biotyping: At line monitoring of recombinant CHO cell cultures

Intact cell MALDI mass spectrometry biotyping for "at-line" monitoring of apoptosis progression in CHO cell cultures

Background Mammalian cell cultures, especially Chinese Hamster Ovary (CHO), are the predominant host for the production of biologics. Despite considerable progress in industry and academia alike (also enforced e.g. by the Process Analytical Technology Initiative of the FDA), particularly in the field of process monitoring there is still a need for innovative methods enabling improvement of proc...

Fish authentication by MALDI-TOF mass spectrometry.

Recent EU directives and regulations for quality control and authentication of food products have prompted the development of new methods for large-scale tests to ensure the protection of consumers. In view of this, an innovative method based on MALDI-TOF mass spectrometry has been developed and successfully applied to fish authentication. Highly specific mass spectrometric profiles from 25 dif...

Rapid identification of mycobacteria from positive MGIT broths of primary cultures by MALDI-TOF mass spectrometry

BACKGROUND Rapid identification of mycobacteria is important for timely treatment and the implementation of public health measures. The MGIT system ensures rapid detection of mycobacteria, but identification is usually delayed by days to weeks due to further subculture on solid medium. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to effective...

Biotyping Saccharomyces cerevisiae strains using Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS)

Routine identity confirmation of recombinant proteins by MALDI-TOF mass spectrometry.

Peptide mass fingerprinting (PMF) by matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides a simple and direct means to unequivocally confirm identity of recombinant proteins based on predicted peptide profiles. Many universities or research institutions now carry mass spectrometry instrumentation as part of their core bioanalytical facilities or p...